SLC6A6/TAUT Polyclonal antibody

SLC6A6/TAUT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26725-1-AP

Synonyms

SLC6A6, TAUT

---

Validation Data Gallery

• 

  WB analysis using 26725-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 26725-1-AP (SLC6A6/TAUT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat eye using 26725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 26725-1-AP (SLC6A6/TAUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 26725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 26725-1-AP (SLC6A6/TAUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IHC detected in: rat eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26725-1-AP targets SLC6A6/TAUT in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC6A6/TAUT fusion protein Ag24935
• Full Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC137128
• Gene Symbol: SLC6A6
• Gene ID (NCBI): 6533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC6A6, also known as TAUT, belongs to the solute carrier 6 (SLC6) family. SLC6A6 is expressed in retinal capillary endothelial cells and mediates the transport of taurine. SLC6A6 can also mediate the transport of beta-alanine, hypotaurine, and gamma-aminobutyric acid. SLC6A6 has 2 isoforms with the molecular mass of 23 and 70 kDa. Sometimes bands of 50 (non-glycosylated) and 75 kDa (glycosylated) may be detected refer to literature (PMID: 35050188).