SLC4A8 Polyclonal antibody

SLC4A8 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12531-1-AP

Synonyms

k NBC3, KIAA0739, NBC, NBC3, NDCBE1, SLC4A8

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Validation Data Gallery

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  WB analysis of human brain using 12531-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12531-1-AP (SLC4A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12531-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12531-1-AP (SLC4A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12531-1-AP targets SLC4A8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC4A8 fusion protein Ag3227
• Full Name: solute carrier family 4, sodium bicarbonate cotransporter, member 8
• Calculated Molecular Weight: 691 aa, 77 kDa
• Observed Molecular Weight: 121 kDa
• GenBank Accession Number: BC025994
• Gene Symbol: SLC4A8
• Gene ID (NCBI): 9498
• RRID: AB_10640898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali
mouse	WB	Cell Metab; Mineralocorticoid receptor phosphorylation regulates ligand binding and renal response to volume depletion and hyperkalemia.; Authors - Shibata Shigeru S
mouse	WB	Am J Physiol Renal Physiol; Effects of extreme potassium stress on blood pressure and renal tubular sodium transport.; Authors - Cary R Boyd-Shiwarski