SLC29A3 Recombinant antibody

SLC29A3 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230281C12

Cat no : 83036-1-RR

Synonyms

ENT3, FLJ11160, hENT3, SLC29A3

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Validation Data Gallery

• 

  WB analysis of human plasma using 83036-1-RR

  human plasma were subjected to SDS PAGE followed by western blot with 83036-1-RR (SLC29A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 83036-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SLC29A3 (83036-1-RR, Clone:230281C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human plasma
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83036-1-RR targets SLC29A3 in WB, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC29A3 fusion protein Ag18563
• Full Name: solute carrier family 29 (nucleoside transporters), member 3
• Calculated Molecular Weight: 475 aa, 52 kDa
• Observed Molecular Weight: 51 kDa, 60 kDa
• GenBank Accession Number: BC120996
• Gene Symbol: SLC29A3
• Gene ID (NCBI): 55315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.