FATP4 Polyclonal antibody

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• KD/KO Validated

FATP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11013-1-AP

Synonyms

ACSVL4, FATP 4, FATP4, Fatty acid transport protein 4, SLC27A4

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Validation Data Gallery

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  WB analysis of HepG2 using 11013-1-AP

  WB result of FATP4 antibody (11013-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FATP4 transfected HepG2 cells.

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  WB analysis of human colon using 11013-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11013-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11013-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 11013-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11013-1-AP

  IP result of anti-FATP4 (IP:11013-1-AP, 4ug; Detection:11013-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11013-1-AP (FATP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, human colon tissue, human heart tissue, SMMC-7721 cells, mouse colon tissue, rat colon tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11013-1-AP targets FATP4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FATP4 fusion protein Ag1490
• Full Name: solute carrier family 27 (fatty acid transporter), member 4
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC009959
• Gene Symbol: SLC27A4
• Gene ID (NCBI): 10999
• RRID: AB_2190637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Sci Rep; PMID: 28304381; Authors - Wenjing Li
human	WB	Front Immunol; Cathelicidin-WA Facilitated Intestinal Fatty Acid Absorption Through Enhancing PPAR-γ Dependent Barrier Function.; Authors - Xin Zong
mouse	WB	iScience; Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.; Authors - Hu Hua
	WB	Int J Mol Sci; Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco.; Authors - Shi-Cheng Ling
mouse	IHC	Biochim Biophys Acta; Uninephrectomy augments the effects of high fat diet induced obesity on gene expression in mouse kidney.; Authors - Zhibo Gai
mouse,pig	WB	Cells; LPS Inhibits Fatty Acid Absorption in Enterocytes through TNF-α Secreted by Macrophages.; Authors - Heyuan Liu