SKIV2L2 Polyclonal antibody

SKIV2L2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12719-2-AP

Synonyms

ATP dependent helicase SKIV2L2, Dob1, fSAP118, KIAA0052, Mtr4, SKIV2L2

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Validation Data Gallery

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12719-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12719-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12719-2-AP

  IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:300) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

12719-2-AP targets SKIV2L2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SKIV2L2 fusion protein Ag3414
• Full Name: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 1042 aa, 118 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC028604
• Gene Symbol: SKIV2L2
• Gene ID (NCBI): 23517
• RRID: AB_2187479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IP	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan