CoraLite® Plus 488-conjugated SKAP2 Monoclonal antibody

SKAP2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G3G9

Cat no : CL488-66367

Synonyms

PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL488-66367

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 SKAP2 antibody (CL488-66367, Clone: 2G3G9 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL488-66367

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SKAP2 (CL488-66367, Clone:2G3G9) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66367 targets SKAP2 in IF, FC (Intra) applications and shows reactivity with human, pig, rat, mouse samples.

• Tested Reactivity: human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SKAP2 fusion protein Ag3615
• Full Name: src kinase associated phosphoprotein 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC036044
• Gene Symbol: SKAP2
• Gene ID (NCBI): 8935
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.