SIRT3 Recombinant antibody

SIRT3 Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2P6

Cat no : 82210-1-RR

Synonyms

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3

---

Validation Data Gallery

• 

  WB analysis using 82210-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82210-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 82210-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 82210-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, rat heart tissue, HepG2 cells, HT-29 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82210-1-RR targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SIRT3 fusion protein Ag26280
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC001042
• Gene Symbol: SIRT3
• Gene ID (NCBI): 23410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.