SIRPD Polyclonal antibody

SIRPD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26594-1-AP

Synonyms

PTPNS1L2, SIRP delta, SIRPD

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Validation Data Gallery

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  WB analysis of fetal human brain using 26594-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26594-1-AP (SIRPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 26594-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26594-1-AP (SIRPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 26594-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26594-1-AP (SIRPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26594-1-AP is SIRPD Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRPD fusion protein Ag24246
• Full Name: signal-regulatory protein delta
• Calculated Molecular Weight: 197 aa, 22 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC033502
• Gene Symbol: SIRPD
• Gene ID (NCBI): 128646
• RRID: AB_2880567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.