SIP1 Polyclonal antibody

SIP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 21672-1-AP

Synonyms

Component of gems 2, Gemin 2, GEMIN2, SIP1, SIP1 delta, SMN interacting protein 1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, rat brain tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 21672-1-AP is SIP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIP1 fusion protein Ag16500
Full Name survival of motor neuron protein interacting protein 1
Calculated Molecular Weight 280 aa, 32 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC104968
Gene Symbol SIP1
Gene ID (NCBI) 8487
RRIDAB_10859249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SIP1 antibody 21672-1-APDownload protocol
IP protocol for SIP1 antibody 21672-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.

Authors - Alessia Mirra
human

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi
humanWB

J Mol Med (Berl)

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Authors - Yuhong Zhang
humanWB

Biomolecules

(20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2.

Authors - Yu-Shi Wang