CoraLite® Plus 488-conjugated SIAE Monoclonal antibody

SIAE Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat , pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G5F7

Cat no : CL488-66522

Synonyms

CSE C, H Lse, LSE, SIAE, Sialate O acetylesterase, sialic acid acetylesterase, YSG2

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Validation Data Gallery

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  FC experiment of PC-3 using CL488-66522

  1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SIAE (CL488-66522, Clone:1G5F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: PC-3 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66522 targets SIAE in FC (Intra) applications and shows reactivity with Human, mouse , rat , pig samples.

• Tested Reactivity: Human, mouse , rat , pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIAE fusion protein Ag14624
• Full Name: sialic acid acetylesterase
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC068450
• Gene Symbol: SIAE
• Gene ID (NCBI): 54414
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.