SHPK Recombinant antibody
SHPK Recombinant Antibody for IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
230115A4
Cat no : 82875-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | U2OS cells |
Positive FC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:150-1:600 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82875-1-RR targets SHPK in IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SHPK fusion protein Ag33893 |
Full Name | sedoheptulokinase |
Calculated Molecular Weight | 478 aa, 52 kDa |
GenBank Accession Number | BC020543 |
Gene Symbol | SHPK |
Gene ID (NCBI) | 23729 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).
Protocols
Product Specific Protocols | |
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IF protocol for SHPK antibody 82875-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |