SHKBP1 Polyclonal antibody

SHKBP1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12703-1-AP

Synonyms

PP203, Sb1, SH3KBP1 binding protein 1, SHKBP1

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Validation Data Gallery

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  WB analysis of A431 using 12703-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12703-1-AP (SHKBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 12703-1-AP

  Immunofluorescent analysis of HepG2 cells, using SHKBP1 antibody 12703-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12703-1-AP targets SHKBP1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHKBP1 fusion protein Ag3386
• Full Name: SH3KBP1 binding protein 1
• Calculated Molecular Weight: 707 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC022855
• Gene Symbol: SHKBP1
• Gene ID (NCBI): 92799
• RRID: AB_10637860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.