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  • KD/KO Validated

SH3BP4 Polyclonal antibody

SH3BP4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17691-1-AP

Synonyms

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17691-1-AP targets SH3BP4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3BP4 fusion protein Ag11847
Full Name SH3-domain binding protein 4
Calculated Molecular Weight 963 aa, 108 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC057396
Gene Symbol SH3BP4
Gene ID (NCBI) 23677
RRIDAB_2189364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SH3BP4 antibody 17691-1-APDownload protocol
IF protocol for SH3BP4 antibody 17691-1-APDownload protocol
IP protocol for SH3BP4 antibody 17691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Mol Med

SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF.

Authors - Kyu-Han Kim
  • KD Validated
pigWB

J Appl Physiol (1985)

Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs.

Authors - Agus Suryawan

Nat Commun

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival

Authors - Joanne Watson