SH3BGRL3 Polyclonal antibody

SH3BGRL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12210-1-AP

Synonyms

SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1

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Validation Data Gallery

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  WB analysis using 12210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 12210-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12210-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, U-251 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12210-1-AP targets SH3BGRL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3BGRL3 fusion protein Ag2857
• Full Name: SH3 domain binding glutamic acid-rich protein like 3
• Calculated Molecular Weight: 93 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC030135
• Gene Symbol: SH3BGRL3
• Gene ID (NCBI): 83442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein, could act as a modulator of glutaredoxin biological activity. It may play a role in cytoskeleton organization (PMID: 34380438). SH3BGRL3 acts as a potential prognostic biomarker for urothelial carcinoma and a novel binding partner of epidermal growth factor receptor (PMID: 26286913).

Publications

Species	Application	Title
human	WB	ACS Chem Biol; Citrullination/Methylation Crosstalk on Histone H3 Regulates ER-Target Gene Transcription.; Authors - Kathleen W Clancy