SGSM3 Polyclonal antibody

SGSM3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20825-1-AP

Synonyms

MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3

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Validation Data Gallery

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  WB analysis of fetal human brain using 20825-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20825-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20825-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20825-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20825-1-AP

  IP result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:600) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human heart using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 20825-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20825-1-AP (SGSM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20825-1-AP

  Immunofluorescent analysis of Hela cells, using SGSM3 antibody 20825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, L02 cells, mouse brain tissue, mouse testis tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

The immunogen of 20825-1-AP is SGSM3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGSM3 fusion protein Ag14800
• Full Name: small G protein signaling modulator 3
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC008078
• Gene Symbol: SGSM3
• Gene ID (NCBI): 27352
• RRID: AB_10693680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Sci Rep; AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.; Authors - Ermes Candiello
mouse	WB	Mediators Inflamm; Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling.; Authors - Yuyi Huang
mouse	WB	Cell Rep; Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.; Authors -
mouse	WB	J Cell Biol; TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1; Authors - Shu Hiragi ; KD Validated