SGSH Polyclonal antibody

SGSH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14184-1-AP

Synonyms

HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase

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Validation Data Gallery

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  WB analysis of HEK-293 using 14184-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14184-1-AP (SGSH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14184-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14184-1-AP (SGSH Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IHC staining of human pancreas cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Transfected
• Positive IHC detected in: human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14184-1-AP targets SGSH in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGSH fusion protein Ag5435
• Full Name: N-sulfoglucosamine sulfohydrolase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC047318
• Gene Symbol: SGSH
• Gene ID (NCBI): 6448
• RRID: AB_2270366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SGSH(N-sulphoglucosamine sulphohydrolase) is also named as HSS, sulphamidase and belongs to the sulfatase family. Sulfamidase is synthesized as a 62 kDa precursor protein, which is modified with mannose 6-phosphate (M6P) residues, allowing their recognition by mannose-6-phosphate receptors in the Golgi complex and ensuring transport to the endosomal/lysosomal system(PMID:21671382). It catalyzes the third step of degradation of glucosaminoglycans and is required for the degradation of heparan sulphate. Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A), also known as Sanfilippo syndrome A.