SGK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SGK3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12699-1-AP

Synonyms

CISK, SGK3, SGKL

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Validation Data Gallery

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  WB analysis of HeLa using 12699-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 12699-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12699-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 12699-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12699-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12699-1-AP

  IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human pancreas cancer using 12699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12699-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 12699-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A375 using 12699-1-AP

  Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, human liver tissue, mouse skin tissue, rat kidney tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human pancreas cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12699-1-AP targets SGK3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGK3 fusion protein Ag3149
• Full Name: serum/glucocorticoid regulated kinase family, member 3
• Calculated Molecular Weight: 496 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC015326
• Gene Symbol: SGK3
• Gene ID (NCBI): 23678
• RRID: AB_2188548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.

Publications

Species	Application	Title
mouse	WB	Autophagy; Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.; Authors - Seonghee Jung ; KO Validated
mouse	IF	Front Cell Dev Biol; Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.; Authors - Kang Guo
mouse,rat	WB,IHC,IF	Front Pharmacol; The role of the SGK3/TOPK signaling pathway in the transition from acute kidney injury to chronic kidney disease; Authors - Huapan Shu ; KD Validated