SGEF Polyclonal antibody

SGEF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12493-1-AP

Synonyms

CSGEF, HMFN1864, SGEF



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

12493-1-AP targets SGEF in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGEF fusion protein Ag3198
Full Name Src homology 3 domain-containing guanine nucleotide exchange factor
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC016628
Gene Symbol SGEF
Gene ID (NCBI) 26084
RRIDAB_10896997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SGEF antibody 12493-1-APDownload protocol
IHC protocol for SGEF antibody 12493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

PLoS One

The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis.

Authors - Samson Thomas T