SFXN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFXN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12296-1-AP

Synonyms

SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein

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Validation Data Gallery

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  WB analysis using 12296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12296-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12296-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12296-1-AP

  IP result of anti-SFXN1 (IP:12296-1-AP, 4ug; Detection:12296-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: MCF-7 cells, human brain tissue, mouse liver tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12296-1-AP targets SFXN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFXN1 fusion protein Ag2985
• Full Name: sideroflexin 1
• Calculated Molecular Weight: 35.6 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC020517
• Gene Symbol: SFXN1
• Gene ID (NCBI): 94081
• RRID: AB_2185814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.; Authors - Mariluz Soula ; KO Validated
mouse	IHC,IF,IP	J Proteome Res; The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.; Authors - Fowler Stephanie L SL
human	WB	Cell Rep; The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.; Authors - Michelle Grace Acoba ; KO Validated
mouse	WB	Front Immunol; YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis; Authors - Jing Zhang
human	IHC,WB	Sci Rep; Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma; Authors - Kohei Yagi
mouse,rat	IHC,WB	Sci Total Environ; Ferritinophagy activation and sideroflexin1-dependent mitochondrial iron overload contribute to patulin-induced cardiac inflammation and fibrosis; Authors - Wen-Chang Sun ; KD Validated