SFRS11 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFRS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26751-1-AP

Synonyms

p54, SFRS11, SRSF11

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Validation Data Gallery

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  WB analysis of HEK-293T using 26751-1-AP

  WB result of SFRS11 antibody (26751-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFRS11 transfected HEK-293T cells.

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  WB analysis using 26751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26751-1-AP (SFRS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human breast cancer using 26751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of rat colon using 26751-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 26751-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IHC detected in: human breast cancer tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26751-1-AP targets SFRS11 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRS11 fusion protein Ag25015
• Full Name: splicing factor, arginine/serine-rich 11
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC040436
• Gene Symbol: SFRS11
• Gene ID (NCBI): 9295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.