SFRP5 Polyclonal antibody

SFRP5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14283-1-AP

Synonyms

Frizzled related protein 1b, FRP 1b, FRP1B, SARP 3, SARP3, sFRP 5, SFRP5

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Validation Data Gallery

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  WB analysis using 14283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14283-1-AP (SFRP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 14283-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14283-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 14283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14283-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat eye using 14283-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14283-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14283-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14283-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 14283-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP5 antibody (14283-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HUVEC cells, L02 cells, mouse heart tissue, mouse retina tissue
• Positive IHC detected in: human thyroid cancer tissue, human cervical cancer tissue, mouse heart tissue, rat eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14283-1-AP targets SFRP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRP5 fusion protein Ag5555
• Full Name: secreted frizzled-related protein 5
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36-42 kDa
• GenBank Accession Number: BC050435
• Gene Symbol: SFRP5
• Gene ID (NCBI): 6425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secreted frizzled-related protein 5 (SFRP5) is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes. SFRP5 is secreted by adipocytes and that it controls the micro-environment of white adipose tissue under conditions of metabolic stress (PMID: 20558665). SFRP5 gene consists of 317 amino acid residues and 3 coding exons and maps to chromosome 10q24.1 in humans. SFRP5 was initially found to be highly expressed in retinal pigment epithelium cells and moderately expressed in the pancreas. SFRP5 is involved in the pathological process of obesity, insulin resistance, dyslipidemia, inflammation, and fibrosis and is associated with obesity, T2D, and ASCVD (PMID: 31199714, PMID: 32004418).