SFRP4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFRP4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15328-1-AP

Synonyms

FRP 4, FRPHE, sFRP 4, SFRP4

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Validation Data Gallery

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  WB analysis of HEK-293 using 15328-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15328-1-AP

  IP result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human skin cancer tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

15328-1-AP targets SFRP4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRP4 fusion protein Ag6909
• Full Name: secreted frizzled-related protein 4
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 41-51 kDa, 55 kDa
• GenBank Accession Number: BC058911
• Gene Symbol: SFRP4
• Gene ID (NCBI): 6424
• RRID: AB_2187099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.

Publications

Species	Application	Title
human	IHC	Nat Commun; Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin.; Authors - Tracy Tabib
human	IHC	Sci Rep; Expression profiling of prostate cancer tissue delineates genes associated with recurrence after prostatectomy.; Authors - Martin Mørck Mortensen
mouse	WB	Cell Physiol Biochem; Differential Patterns of Secreted Frizzled-Related Protein 4 (SFRP4) in Adipocyte Differentiation: Adipose Depot Specificity.; Authors - Hua Guan ; KD Validated
human,mouse	WB,IHC	Gene Ther; Effect of nanoparticle-mediated delivery of SFRP4 siRNA for treating Dupuytren disease.; Authors - Rujue Jin ; KD Validated
human	IHC	Sci Rep; SFRP4 gene expression is increased in aggressive prostate cancer.; Authors - Elise Sandsmark
mouse	WB	Endocrinology; SFRP4 Is a Negative Regulator of Ovarian Follicle Development and Female Fertility.; Authors - Gustavo Zamberlam ; KO Validated