SF1 Polyclonal antibody

SF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11635-1-AP

Synonyms

BBP, D11S636, mBBP, SF1, splicing factor 1, Transcription factor ZFM1, ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162

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Validation Data Gallery

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  WB analysis using 11635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 11635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 11635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11635-1-AP targets SF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF1 fusion protein Ag2209
• Full Name: splicing factor 1
• Calculated Molecular Weight: 638 aa, 68 kDa
• Observed Molecular Weight: 65-71 kDa
• GenBank Accession Number: BC020217
• Gene Symbol: SF1
• Gene ID (NCBI): 7536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.

Publications

Species	Application	Title
	WB	Retrovirology; A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.; Authors - Rebecca Moschall
human	IHC,IF	Cancers (Basel); Targeting Oncogenic Wnt/β-Catenin Signaling in Adrenocortical Carcinoma Disrupts ECM Expression and Impairs Tumor Growth; Authors - Morgan K Penny