SETD8 Polyclonal antibody

• Featured Product
• KD/KO Validated

SETD8 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, FC, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 14063-1-AP

Synonyms

H4 K20 HMTase SETD8, KMT5A, Lysine N methyltransferase 5A, PR Set7, PR/SET07, PRSET7, SET07, SET8, SETD8

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Validation Data Gallery

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  WB analysis using 14063-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 14063-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14063-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14063-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293T using 14063-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human SETD8 (14063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, HepG2 cells, A549 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 17 publications below
• IHC: See 11 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Product Information

14063-1-AP targets SETD8 in WB, IP, IF, FC, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SETD8 fusion protein Ag5114
• Full Name: SET domain containing (lysine methyltransferase) 8
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC050346
• Gene Symbol: SETD8
• Gene ID (NCBI): 387893
• RRID: AB_2878006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD8, also known as Set8, PR-Set7, or KMT5A, is a member of the SET domain family of histone lysine methyltransferases and, to date, the only known enzyme that catalyzes monomethylation of H4K20 in eukaryotes, which is involved in DNA damage response, mitotic condensation, and DNA replication. SETD8 is overexpressed in various types of tumors, such as bladder cancer, non-small cell lung carcinoma, and small cell lung carcinoma, and is associated with a shorter survival time for gastric, esophageal, and prostate cancer patients.

Publications

Species	Application	Title
rat	WB,IF	Mol Cell Proteomics; Temporal Proteomic Analysis of Pancreatic β-Cells in Response to Lipotoxicity and Glucolipotoxicity.; Authors - Zonghong Li
human	WB,IHC	Cancer Lett; SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.; Authors - Mengqi Liu
human	WB,IF,CoIP	Oxid Med Cell Longev; Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression.; Authors - Jie Qi ; KD Validated
human,rat	WB,IHC，IF	Exp Mol Med; SET8 suppression mediates high glucose-induced vascular endothelial inflammation via the upregulation of PTEN.; Authors - Xuefang Shen ; KD Validated
human	WB	Cell Biosci; DDB2 regulates DNA replication through PCNA-independent degradation of CDT2.; Authors - Xiaojun Wu
human,rat	WB,IHC,IF,CoIP	Int J Biol Sci; KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy; Authors - Lihong Lu ; KD Validated