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  • KD/KO Validated

SETBP1 Polyclonal antibody

SETBP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16841-1-AP

Synonyms

KIAA0437, SEB, SET binding protein, SET binding protein 1, SETBP1



Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16841-1-AP targets SETBP1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETBP1 fusion protein Ag10532
Full Name SET binding protein 1
Calculated Molecular Weight 175 kDa, 26 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberBC062338
Gene Symbol SETBP1
Gene ID (NCBI) 26040
RRIDAB_2185750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETBP1 is initially found to interact with SET, which is a small protein inhibitor for tumor suppressors PP2A and NM23-H1. There are existing two isoforms of SETBP1 and the molecular weight of isoform one is 170 kDa and another is 26 kDa. SET is fused with another gene CAN through chromosome translocation in a case of acute undifferentiated leukemia. SETBP1 may involve in chromosome translocation in a case of acute T-cell leukemia [PMID:17569777]. SETBP1 can cooperate with BCR/ABL to transform committed myeloid progenitors normally lacking self-renewal capability into LSCs and cause development of myeloid leukemias resembling human CML myeloid blast crisis [PMID:22566606]. SETBP1 was shown to form a complex with SET and PP2A, enhancing the stability of SET and its inhibition of PP2A.

Protocols

Product Specific Protocols
WB protocol for SETBP1 antibody 16841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

Somatic SETBP1 mutations in myeloid malignancies.

Authors - Makishima Hideki H
mouseWB

Blood

Setbp1 promotes the self-renewal of murine myeloid progenitors via activation of Hoxa9 and Hoxa10.

Authors - Oakley Kevin K
  • KD Validated
humanWB,IF,IP

iScience

Recruitment of MLL1 complex is essential for SETBP1 to induce myeloid transformation.

Authors - Nhu Nguyen
human,mouse,canineWB

J Biochem

The protein level of the tumour-promoting factor SET is regulated by cell density.

Authors - Naoki Kohyanagi
  • KD Validated
humanWB

Oncotarget

Myb expression is critical for myeloid leukemia development induced by Setbp1 activation.

Authors - Nhu Nguyen