SERPINA7/TBG Polyclonal antibody

SERPINA7/TBG Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16536-1-AP

Synonyms

Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin

---

Validation Data Gallery

• 

  WB analysis using 16536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16536-1-AP (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16536-1-AP

  IP result of anti-SERPINA7/TBG (IP:16536-1-AP, 4ug; Detection:16536-1-AP 1:500) with HepG2 cells lysate 1080 ug.

---

Tested Applications

• Positive WB detected in: human plasma, human urine
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16536-1-AP targets SERPINA7/TBG in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINA7/TBG fusion protein Ag9823
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• Calculated Molecular Weight: 415 aa, 46 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC020747
• Gene Symbol: SERPINA7
• Gene ID (NCBI): 6906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA7, also known as Thyroxine-binding globulin (TBG), is a 54 kDa acidic glycoprotein, consisting of a single polypeptide chain of 395 amino acids and four oligosaccharide chains. It is one of the major serum transport proteins of thyroid hormones (THs). Although high amounts of albumin are found in serum, TBG has a greater affinity for thyroxine (T4). Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG.