SERPINA10 Monoclonal antibody

SERPINA10 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5B3C9

Cat no : 60113-1-Ig

Synonyms

PZI, Serpin A10, SERPINA10, ZPI

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Validation Data Gallery

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  WB analysis using 60113-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60113-1-Ig (SERPINA10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 60113-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 60113-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 60113-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 60113-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  IF Staining of human liver using 60113-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver using 60113-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, pig liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1500-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

60113-1-Ig targets SERPINA10 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERPINA10 fusion protein Ag2420
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• Calculated Molecular Weight: 444 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC022261
• Gene Symbol: SERPINA10
• Gene ID (NCBI): 51156
• RRID: AB_2301526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA10 belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis.

Publications

Species	Application	Title
human	IHC	Front Oncol; High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.; Authors - Wenwen Guo