SDCCAG8 Monoclonal antibody

SDCCAG8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H1A4

Cat no : 66284-1-Ig

Synonyms

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

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Validation Data Gallery

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  WB analysis of HEK-293 using 66284-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66284-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66284-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66284-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, THP-1 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66284-1-Ig targets SDCCAG8 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDCCAG8 fusion protein Ag4264
• Full Name: serologically defined colon cancer antigen 8
• Calculated Molecular Weight: 713 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC032454
• Gene Symbol: SDCCAG8
• Gene ID (NCBI): 10806
• RRID: AB_2881667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.