SBDS Polyclonal antibody

SBDS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17618-1-AP

Synonyms

CGI 97, SBDS, SDS, SWDS



Tested Applications

Positive WB detected inCaco-2 cells, HL-60 cells, SH-SY5Y cells, HEK-293T cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17618-1-AP targets SBDS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SBDS fusion protein Ag11814
Full Name Shwachman-Bodian-Diamond syndrome
Calculated Molecular Weight 250 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC065700
Gene Symbol SBDS
Gene ID (NCBI) 51119
RRIDAB_2184481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located. This antibody is a rabbit polyclonal antibody raised against a full-length human SBDS protein, recognizes specifically the 29kd SBDS protein.

Protocols

Product Specific Protocols
WB protocol for SBDS antibody 17618-1-APDownload protocol
IHC protocol for SBDS antibody 17618-1-APDownload protocol
IP protocol for SBDS antibody 17618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogenesis

The HIF/PHF8/AR axis promotes prostate cancer progression.

Authors - D Tong
ratWB

Brain Res Bull

Cerebral ischemia-reperfusion causes a down regulation of HCN1 expression via enhancing the nuclear NRSF-HDAC4 gathering that contributes to neuron damage.

Authors - Pan Luo
humanIHC,IF

Am J Transl Res

Three RNA-binding protein signature as a novel biomarker to predict endometrial cancer prognosis

Authors - Bo Wang