SAMD4A Polyclonal antibody

• Featured Product
• KD/KO Validated

SAMD4A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17387-1-AP

Synonyms

hSmaug1, KIAA1053, Protein Smaug homolog 1, SAMD4, SAMD4A, Smaug, Smaug 1, Smaug1, SMG, SMGA

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 17387-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17387-1-AP (SAMD4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17387-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17387-1-AP (SAMD4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 17387-1-AP

  IP result of anti-SAMD4A (IP:17387-1-AP, 4ug; Detection:17387-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human spleen using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human spleen tissue, human kidney tissue, human lung tissue, human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

17387-1-AP targets SAMD4A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMD4A fusion protein Ag11273
• Full Name: sterile alpha motif domain containing 4A
• Calculated Molecular Weight: 529 aa, 59 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC021726
• Gene Symbol: SAMD4A
• Gene ID (NCBI): 23034
• RRID: AB_2301339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Immunol; SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.; Authors - Yuze Wang ; KO Validated
mouse	WB	J Neuroinflammation; Smad7 in the hippocampus contributes to memory impairment in aged mice after anesthesia and surgery; Authors - Changliang Liu
human	IHC,WB	Tissue Cell; SAMD4A serves as a negative prognostic marker for gastric cancer patients; Authors - Deqin Li ; KD Validated