SAAL1 Polyclonal antibody

SAAL1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25467-1-AP

Synonyms

Protein SAAL1, SAAL1, serum amyloid A like 1

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Validation Data Gallery

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  WB analysis of HepG2 using 25467-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25467-1-AP (SAAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25467-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25467-1-AP (SAAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25467-1-AP

  Immunofluorescent analysis of HepG2 cells using 25467-1-AP (SAAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 25467-1-AP is SAAL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAAL1 fusion protein Ag22186
• Full Name: serum amyloid A-like 1
• Calculated Molecular Weight: 475 aa, 54 kDa
• Observed Molecular Weight: 54-55 kDa
• GenBank Accession Number: BC012010
• Gene Symbol: SAAL1
• Gene ID (NCBI): 113174
• RRID: AB_2880094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	BMC Cancer; The effects of the prognostic biomarker SAAL1 on cancer growth and its association with the immune microenvironment in lung adenocarcinoma; Authors - Song Tong
mouse	WB	Front Pharmacol; Bicyclol attenuates high fat diet-induced non-alcoholic fatty liver disease/non-alcoholic steatohepatitis through modulating multiple pathways in mice; Authors - Jingyi Wu