SAA1 Polyclonal antibody

SAA1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16721-1-AP

Synonyms

PIG4, SAA, SAA1, Serum amyloid A protein, serum amyloid A1, TP53I4

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Validation Data Gallery

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  IHC staining of human liver using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human liver tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

16721-1-AP targets SAA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAA1 fusion protein Ag10160
• Full Name: serum amyloid A1
• Calculated Molecular Weight: 122 aa, 14 kDa
• GenBank Accession Number: BC105796
• Gene Symbol: SAA1
• Gene ID (NCBI): 6288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Mol Oncol; Serum amyloid A1 in combination with integrin αVβ3 increases glioblastoma cells mobility and progression.; Authors - Ching-Yu Lin ; KD Validated
human	IHC	J Transl Med; Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model.; Authors - Han Wang

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