RTKN Polyclonal antibody

RTKN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12762-1-AP

Synonyms

rhotekin, RTKN, RTKN1

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Validation Data Gallery

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  WB analysis of A431 using 12762-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12762-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12762-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12762-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12762-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12762-1-AP (RTKN antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12762-1-AP targets RTKN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RTKN fusion protein Ag3265
• Full Name: rhotekin
• Calculated Molecular Weight: 550 aa, 61 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC017727
• Gene Symbol: RTKN
• Gene ID (NCBI): 6242
• RRID: AB_10644159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.