RSPH3 Polyclonal antibody

RSPH3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17603-1-AP

Synonyms

RSHL2, RSP3, RSPH3

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Validation Data Gallery

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  WB analysis of human brain using 17603-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17603-1-AP (RSPH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17603-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17603-1-AP (RSPH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 17603-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 17603-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 17603-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, NIH/3T3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

17603-1-AP targets RSPH3 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSPH3 fusion protein Ag11707
• Full Name: radial spoke 3 homolog (Chlamydomonas)
• Calculated Molecular Weight: 560 aa, 64 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050604
• Gene Symbol: RSPH3
• Gene ID (NCBI): 83861
• RRID: AB_2181073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
		J Mol Cell Biol; Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.; Authors - Yirong Zhang
mouse	IF	Sci Rep; Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice.; Authors - Wenzheng Zou
human	IF	J Assist Reprod Genet; Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3 mutations have a positive pregnancy outcome following intracytoplasmic sperm injection.; Authors - Huan Wu
mouse	CoIP	Nat Commun; Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia; Authors - Xueming Meng
mouse	IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang