RPS5 Polyclonal antibody

RPS5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16964-1-AP

Synonyms

40S ribosomal protein S5, ribosomal protein S5, RPS5

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Validation Data Gallery

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  WB analysis of HeLa using 16964-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16964-1-AP (RPS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16964-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16964-1-AP (RPS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16964-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16964-1-AP (RPS5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16964-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16964-1-AP (RPS5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 16964-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16964-1-AP (RPS5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16964-1-AP

  Immunofluorescent analysis of HepG2 cells using 16964-1-AP (RPS5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16964-1-AP targets RPS5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS5 fusion protein Ag10686
• Full Name: ribosomal protein S5
• Calculated Molecular Weight: 204 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC018151
• Gene Symbol: RPS5
• Gene ID (NCBI): 6193
• RRID: AB_2180894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Nat Struct Mol Biol; Rps26 directs mRNA-specific translation by recognition of Kozak sequence elements.; Authors - Max B Ferretti
human	WB	J Cell Biol; Ribosome biogenesis factor Ltv1 chaperones the assembly of the small subunit head.; Authors - Jason C Collins
	WB	Elife; A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.; Authors - Sabina Schütz
rat	WB	J Biochem Mol Toxicol; Matrine alleviates hypoxia-induced inflammation and pulmonary vascular remodelling via RPS5/NF-κB signalling pathway; Authors - Mingxing Li