RPL9 Monoclonal antibody, PBS Only

RPL9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6B11

Cat no : 68054-1-PBS

Synonyms

60S ribosomal protein L9, DKFZp313J1510, FLJ27456, ribosomal protein L9, RPL9, RPL9P7, RPL9P8, RPL9P9

---

Validation Data Gallery

• 

  WB analysis using 68054-1-Ig (same clone as 68054-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

---

Product Information

68054-1-PBS targets RPL9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPL9 fusion protein Ag5391
• Full Name: ribosomal protein L9
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC066318
• Gene Symbol: RPL9
• Gene ID (NCBI): 6133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.