RPL26L1 Polyclonal antibody

RPL26L1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16487-1-AP

Synonyms

ribosomal protein L26 like 1, RPL26L1, RPL26P1

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Validation Data Gallery

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  WB analysis of HEK-293 using 16487-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16487-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16487-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16487-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16487-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16487-1-AP (RPL26L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, MCF-7 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16487-1-AP targets RPL26L1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL26L1 fusion protein Ag9609
• Full Name: ribosomal protein L26-like 1
• Calculated Molecular Weight: 145 aa, 17 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC017360
• Gene Symbol: RPL26L1
• Gene ID (NCBI): 51121
• RRID: AB_2181740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL26L1, also named as RPL26P1, belongs to the universal ribosomal protein uL24 family. RPL26L1 shares high sequence similarity with ribosomal protein L26 and plays a role in protein synthesis (PMID: 31673979). The molecular mass of RPL26L1 is 17 kDa. Studies have shown that RPL26L1 is related to autism spectrum disorder and is one of the potential biomarkers of the neurodegenerative disease Friedreich's ataxia. In addition, RPL26L1 may be closely related with SLE development (PMID: 31889427).

Publications

Species	Application	Title
rat	WB	Brain Sci; Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest; Authors - Qiang Zhang