RPL23A Polyclonal antibody

RPL23A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16386-1-AP

Synonyms

60S ribosomal protein L23a, MDA20, ribosomal protein L23a, RPL23A

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Validation Data Gallery

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  WB analysis using 16386-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16386-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16386-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16386-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16386-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16386-1-AP

  IP result of anti-RPL23A (IP:16386-1-AP, 4ug; Detection:16386-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human liver using 16386-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16386-1-AP (RPL23A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16386-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16386-1-AP (RPL23A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16386-1-AP

  Immunofluorescent analysis of HepG2 cells using 16386-1-AP (RPL23A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue, human heart tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• RIP: See 1 publications below

Product Information

16386-1-AP targets RPL23A in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL23A fusion protein Ag9424
• Full Name: ribosomal protein L23a
• Calculated Molecular Weight: 156 aa, 18 kDa
• Observed Molecular Weight: 18-23 kDa
• GenBank Accession Number: BC014459
• Gene Symbol: RPL23A
• Gene ID (NCBI): 6147
• RRID: AB_2269755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
mouse	WB,RIP	Nat Commun; The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis.; Authors - Mehdi Pirouz
human	WB	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič
mouse	WB	Gene; Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.; Authors - Trieu-Duc Vu
human	WB	Cell Death Dis; HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer.; Authors - Zhiyuan Zhang