RP9 Polyclonal antibody

RP9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16832-1-AP

Synonyms

PAP 1, Pim 1 associated protein, Retinitis pigmentosa 9 protein, RP9

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Validation Data Gallery

• 

  WB analysis of HeLa using 16832-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16832-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16832-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16832-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16832-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16832-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 16832-1-AP is RP9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RP9 fusion protein Ag10441
• Full Name: retinitis pigmentosa 9 (autosomal dominant)
• Calculated Molecular Weight: 221 aa, 26 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC025928
• Gene Symbol: RP9
• Gene ID (NCBI): 6100
• RRID: AB_2878322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.; Authors - Chang Cui
human	WB	Mol Cell; Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.; Authors - Karl Bertram