ROBO3-Specific Polyclonal antibody

ROBO3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20220-1-AP

Synonyms

HGPPS, HGPS, RBIG1, RIG1, ROBO3, ROBO3-Specific, Roundabout homolog 3, Roundabout like protein 3

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Validation Data Gallery

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  WB analysis using 20220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20220-1-AP (ROBO3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

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  IHC staining of human gliomas using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

20220-1-AP targets ROBO3-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: roundabout, axon guidance receptor, homolog 3 (Drosophila)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: NM_022370
• Gene Symbol: ROBO3
• Gene ID (NCBI): 64221
• RRID: AB_10732825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. The antibody is specific to ROBO3.

Publications

Species	Application	Title
human	IF	J Biol Chem; Non-redundant roles of DIAPHs in primary ciliogenesis.; Authors - Oliva Palander