RNF38 Polyclonal antibody

RNF38 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25132-1-AP

Synonyms

ring finger protein 38, RNF38

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Validation Data Gallery

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  WB analysis of HepG2 using 25132-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25132-1-AP (RNF38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 25132-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25132-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25132-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25132-1-AP (RNF38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 25132-1-AP is RNF38 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF38 fusion protein Ag16054
• Full Name: ring finger protein 38
• Calculated Molecular Weight: 515 aa, 58 kDa
• Observed Molecular Weight: 43-58 kDa
• GenBank Accession Number: BC033786
• Gene Symbol: RNF38
• Gene ID (NCBI): 152006
• RRID: AB_2879914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RING finger protein 38 is a protein that in humans is encoded by the RNF38 gene.This gene encodes a protein with a coiled-coil motif and a RING-H2 motif (C3H2C2) at its carboxy-terminus. The RING motif is a zinc-binding domain found in a large set of proteins playing roles in diverse cellular processes including oncogenesis, development, signal transduction, and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RNF38 is widely expressed with highest levels in testis. RNF38 exists various isoforms and molecular weight of isoforms are 36, 48, 52 and 58 kDa

Publications

Species	Application	Title
human	WB,IHC	J Cancer; Ring finger protein 38 promote non-small cell lung cancer progression by endowing cell EMT phenotype.; Authors - Dian Xiong