RNF181 Polyclonal antibody

RNF181 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20408-1-AP

Synonyms

HSPC238, ring finger protein 181, RN181, RNF181

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Validation Data Gallery

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  WB analysis of Jurkat using 20408-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20408-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20408-1-AP

  IP result of anti-RNF181 (IP:20408-1-AP, 3ug; Detection:20408-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human kidney using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20408-1-AP

  Immunofluorescent analysis of HepG2 cells, using RNF181 antibody 20408-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, L02 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 20408-1-AP is RNF181 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF181 fusion protein Ag14233
• Full Name: ring finger protein 181
• Calculated Molecular Weight: 153 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC002803
• Gene Symbol: RNF181
• Gene ID (NCBI): 51255
• RRID: AB_10696314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Western blot analysis detected RNF181 protein in human platelets as a 36-kD dimer and an 18-kD monomer.

Publications

Species	Application	Title
	WB	Oncol Rep; Screening and verification of proteins that interact with HSPC238.; Authors - Jia-Yu Tan