RNF141 Polyclonal antibody

RNF141 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26041-1-AP

Synonyms

ring finger protein 141, RNF141, ZFP26, Zinc finger protein 230, ZNF230

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Validation Data Gallery

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  WB analysis of mouse testis using 26041-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26041-1-AP (RNF141 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse testis using 26041-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26041-1-AP (RNF141 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 26041-1-AP

  IP result of anti-RNF141 (IP:26041-1-AP, 4ug; Detection:26041-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human colon using 26041-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26041-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26041-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26041-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26041-1-AP is RNF141 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF141 fusion protein Ag23316
• Full Name: ring finger protein 141
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC018104
• Gene Symbol: RNF141
• Gene ID (NCBI): 50862
• RRID: AB_2880348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.