RNF133 Polyclonal antibody

RNF133 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20792-1-AP

Synonyms

ring finger protein 133, RNF133

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Validation Data Gallery

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  WB analysis of HeLa using 20792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20792-1-AP (RNF133 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 20792-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20792-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20792-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20792-1-AP (RNF133 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20792-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20792-1-AP (RNF133 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat testis using 20792-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20792-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20792-1-AP

  Immunofluorescent analysis of Hela cells, using RNF133 antibody 20792-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, human brain tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20792-1-AP is RNF133 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF133 fusion protein Ag14537
• Full Name: ring finger protein 133
• Calculated Molecular Weight: 376 aa, 42 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC022038
• Gene Symbol: RNF133
• Gene ID (NCBI): 168433
• RRID: AB_10695189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.