RIPK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RIPK2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 15366-1-AP

Synonyms

CARD3, CARDIAK, CCK, GIG30, Receptor interacting protein 2, RICK, RIP 2, RIP2, RIPK2

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Validation Data Gallery

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  WB analysis using 15366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15366-1-AP (RIPK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15366-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15366-1-AP (RIPK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 15366-1-AP

  IP result of anti-RIPK2 (IP:15366-1-AP, 4ug; Detection:15366-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, HeLa cells, THP-1 cells
• Positive IP detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

15366-1-AP targets RIPK2 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIPK2 fusion protein Ag0909
• Full Name: receptor-interacting serine-threonine kinase 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 46-55 kDa, 61-72 kDa
• GenBank Accession Number: BC004553
• Gene Symbol: RIPK2
• Gene ID (NCBI): 8767
• RRID: AB_2253650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
pig	WB	Antioxidants (Basel); M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide; Authors - Menghui Xu
human	WB	Obesity (Silver Spring); Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.; Authors - Yanping Wang
human	WB	J Pharmacol Exp Ther; Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.; Authors - Mohamed Salla ; KD Validated
mouse	WB	Front Cell Infect Microbiol; Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.; Authors - Chun-Xia Shi
mouse	WB,IP	Immunol Lett; A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation.; Authors - Dong-Yi Cao
human	WB	Front Genet; Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.; Authors - Qian Yang ; KD Validated