RIOK1 Polyclonal antibody

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• KD/KO Validated

RIOK1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17222-1-AP

Synonyms

AD034, RIO kinase 1, RIO kinase 1 (yeast), RIOK1

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Validation Data Gallery

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  WB analysis of HeLa using 17222-1-AP

  WB result of RIOK1 antibody (17222-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIOK1 transfected HeLa cells.

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  WB analysis of HeLa using 17222-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 17222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17222-1-AP

  IP result of anti-RIOK1 (IP:17222-1-AP, 4ug; Detection:17222-1-AP 1:500) with HeLa cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17222-1-AP targets RIOK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIOK1 fusion protein Ag11057
• Full Name: RIO kinase 1 (yeast)
• Calculated Molecular Weight: 568 aa, 66 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC006104
• Gene Symbol: RIOK1
• Gene ID (NCBI): 83732
• RRID: AB_2284990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biomedicines; RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.; Authors - Min Yu
human	WB	iScience; Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.; Authors - Laura S Bisogno
human	WB	Cancer Res Treat; Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression.; Authors - Zhiqi Huang