CoraLite® Plus 488-conjugated RIC8A Monoclonal antibody

RIC8A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3B11

Cat no : CL488-66625

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-66625

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 RIC8A antibody (CL488-66625, Clone: 1E3B11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66625

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 RIC8A antibody (CL488-66625, Clone: 1E3B11 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66625 targets RIC8A in IF applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RIC8A fusion protein Ag1593
• Full Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011821
• Gene Symbol: RIC8A
• Gene ID (NCBI): 60626
• RRID: AB_2919352
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.