RHOF Polyclonal antibody

RHOF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12290-1-AP

Synonyms

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

---

Validation Data Gallery

• 

  WB analysis using 12290-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12290-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12290-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12290-1-AP

  IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug.

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12290-1-AP targets RHOF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOF fusion protein Ag2943
• Full Name: ras homolog gene family, member F (in filopodia)
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC018208
• Gene Symbol: RHOF
• Gene ID (NCBI): 54509
• RRID: AB_10644126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression; Authors - Michiru Nishita
human	WB	Int J Mol Sci; An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer; Authors - Yongcheng Su