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  • KD/KO Validated

RHBDD1 Polyclonal antibody

RHBDD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20869-1-AP

Synonyms

HSD 50, RHBDD1, RHBDL4, rhomboid domain containing 1



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, mouse testis tissue, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20869-1-AP targets RHBDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHBDD1 fusion protein Ag14932
Full Name rhomboid domain containing 1
Calculated Molecular Weight 315 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC027900
Gene Symbol RHBDD1
Gene ID (NCBI) 84236
RRIDAB_10733756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RHBDD1 antibody 20869-1-APDownload protocol
IHC protocol for RHBDD1 antibody 20869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.

Authors - Koji Shibuya
humanWB

Environ Toxicol

Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway.

Authors - Jiong Jiang
  • KD Validated
humanWB

Cell Death Dis

Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.

Authors - Xiaohui Zhang
humanWB

Genes Cells

Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation

Authors - Shinichiro Yamamoto
  • KD Validated