RGS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RGS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12547-1-AP

Synonyms

1R20, B cell activation protein BL34, BL34, Early response protein 1R20, IER1, IR20, RGS1

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Validation Data Gallery

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  WB analysis using 12547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12547-1-AP

  WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells.

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse small intestine tissue, HepG2 cells, NK-92 cells, rat small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12547-1-AP targets RGS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS1 fusion protein Ag3315
• Full Name: regulator of G-protein signaling 1
• Calculated Molecular Weight: 196 aa, 22 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015510
• Gene Symbol: RGS1
• Gene ID (NCBI): 5996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).